Research Paper Volume 16, Issue 4 pp 3647—3673

Figure 12. FLRT3 overexpression inhibits the proliferation and metastasis of ccRCC cells. (A, B) The Western blot experiment demonstrated that FLRT3 was overexpressed in the OE group. (C) CCK8 assay showed that FLRT3 overexpression led to reduced proliferation capacity of A498 and 786-O cells. (D, E) The results from EdU incorporation experiments showed that FLRT3 overexpression resulted in a significant reduction in the proliferation capacity of A498 and 786-O cells. (F, G) The transwell assay illustrates that FLRT3 overexpression significantly impairs the invasive ability of A498 and 786-O cells. (H, I) Wound healing assays illustrated that elevated FLRT3 expression caused a decrease in the migratory capacity of A498 and 786-O cells. Scale bar: 500 μm.