Research Paper Volume 16, Issue 4 pp 3363—3385

Pan-cancer analysis of NUDT21 and its effect on the proliferation of human head and neck squamous cell carcinoma

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Figure 7. Knockout of NUDT21 inhibits the proliferation of FaDu and CNE-2Z cells. (AD) Measurement of cell proliferation in shCtrl and shNUDT21 treated cells using MTT assay. (E, F) Raw images of shCtrl and shNUDT2-treated cell growth (unprocessed by software algorithms) were obtained by applying Celigo® cytometer imaging analysis. (G, H) shCtrl and shNUDT21-treated cells were inoculated in 96-well plates and cell growth was measured daily for 5 days. (I, J) Cell growth rates were monitored by assays on days 2, 3, 4, and 5. (shCtrl: control lentivirus; shNUDT21: lentivirus containing shRNA targeting NUDT21). ***P<0.001 compared to shCtrl.