Research Paper Volume 16, Issue 4 pp 3363—3385
Pan-cancer analysis of NUDT21 and its effect on the proliferation of human head and neck squamous cell carcinoma
Figure 6. Upregulation of NUDT21 expression levels in HHNSCC and knockdown of NUDT21 by lentivirus-mediated RNAi system. (A–C) NUDT21 protein expression levels as well as gene levels in cancer tissues (CT) and paired adjacent non-tumor tissues (ANT) of 58 pairs of patients from the Second Hospital of Jilin University Cancer Center were counted by immunofluorescence method. Results included 58 pairs of patients (P<0.01) normalized with 18S rRNA. (D) Relative NUDT21 mRNA levels in three head and neck cancer cell lines (FaDu, CNE-2Z and CAL27). (E) Fluorescence photographs of FaDu and CNE-2Z cells infected by lentivirus (100×). (F, G) Identification of NUDT21 knockdown efficiency in FaDu and CNE-2Z cells by RT-PCR. (H) Identification of NUDT21 knockdown efficiency by FLAG protein blotting in FaDu cells. (shCtrl: control lentivirus; shNUDT21: lentivirus containing shRNA targeting NUDT21). **P <0.01 compared to shCtrl.