Research Paper Volume 16, Issue 4 pp 3257—3279

Figure 1. BubR1 inhibits lipid metabolism degradation under starvation. (A) Representative images of Oil red O (ORO) stain of dissected fat bodies from W¹¹¹⁸, BubR1^MI01546, BubR1^k03113 before and after starvation (72 h). (B) Bodipy stain of dissected fat body of W¹¹¹⁸, BubR1^MI01546/BubR1^k03113 before and after starvation (72 h). Bodipy (neutral lipids; green) and Hoechst (Hoechst; blue) detected through fluorescent histochemistry. (C) The dot graph of the mean area of lipid droplets among the more than 30 ROI (region of interest) from W¹¹¹⁸, BubR1^MI01546/BubR1^k03113 before and after starvation (72 h). Each dot corresponds to one ROI. (D) Quantification of total triglyceride (TAG) levels of whole flies in control and BubR1^MI01546/BubR1^k03113 before and after starvation (72 h). n = 9 samples. (E) The total triglyceride (TAG) levels of female flies (W¹¹¹⁸, BubR1^MI01546/BubR1^k03113; r4-Gal4> W¹¹¹⁸ and BubR1^MI01546/BubR1^k03113; r4-Gal4> UAS-BubR1) before and after starvation (72 h), n = 9 samples. (F) Starvation resistance of female flies in W¹¹¹⁸, BubR1^MI01546/BubR1^k03113. n = 4 cohorts (total 80 flies). Data are presented as percents and SE. Scale bars represent 1000 μm (A), 10 μm (B). Without notification, Data are presented as mean and SD. Student’s t-tests were performed. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, and Abbreviation: n.s.: non-significant represents p > 0.05.