Research Paper Volume 16, Issue 3 pp 2908—2933

Figure 1. Identification of anoikis-related genes in NSCLC patients in the TCGA NSCLC cohort. (A) Principal component analysis of TCGA samples based on the expression matrix of anoikis-related genes obtained from the genecards website and the Harmonizome portals. (B) Volcano plot showing the differentially expressed genes (DEGs) for the NSCLC tissues and paracancerous in the TCGA-NSCLC cohort. (C) 33 anoikis-related genes were up-regulated in NSCLC patients in the TCGA cohort. (D) Heatmap showing the expression values of anoikis-related between the NSCLC tissues and paracancerous in the TCGA-NSCLC cohort. (E) The specific location of anoikis-related genes on the human chromosomes. (F) Mutation frequency of 33 overlapping signature genes in the TCGA NSCLC cohort. Each column represented a single patient. The upper bar plot showed TMB. The number on the right indicates the mutation frequency of each regulatory gene. The right bar plot showed the proportion of each variant type. The stacked bar plot below showed a fraction of conversions in each sample. (G) CNV frequency of 33 overlapping genes in the TCGA NSCLC cohort. The height of the column represented the alteration frequency. Blue dot: the deletion frequency; Red dot: the amplification frequency.