Research Paper Volume 16, Issue 3 pp 2005—2025

Figure 5. EC-specific VDR knockdown increases the number of Pros⁺ cells. (A) EC-specific VDR knockdown increases Pros⁺ cell number. Flies carrying the Myo^ts>GFP or Myo^ts>GFP+VDRRi genotypes were incubated at 29° C for 1 week. The whole guts of flies were dissected, fixed, and labeled with anti-Pros (red) and anti-GFP (green) antibodies and DAPI (blue). The original magnification is 200×. (B) Frequency of Pros⁺ cells per total cells. Three-day-old females were shifted to 29° C for 1 week, and their dissected guts were fixed and immunostained with anti-Pros (red) and anti-GFP (green) antibodies and DAPI (blue). The Pros⁺ cell numbers were recorded with respect to the total cells of these guts. Data (mean ± standard error) in Myo^ts>GFP or Myo^ts>GFP+VDRRi flies were collated from 7459 and 7992 total cells of 19 guts each, respectively. P-values were determined using Student’s t-test. P<0.0001 compared with Myo^ts>GFP flies.