Research Paper Volume 16, Issue 3 pp 2828—2847

Figure 7. MiR-124 relieves oxidative stress and apoptosis in HIBD rats by inhibiting STAT3 in vivo. (A) The miR-124 was detected by qRT-PCR; (B) The STAT3 mRNA was detected by qRT-PCR; (C) The protein levels of STAT3 was detected by Western blot; (D) Quantitative analysis of the protein levels of STAT3; (E) The levels of intracellular ROS was measured; (F) The SOD activity was determined by the commercial kits; (G) The content of MDA was determined by the commercial kits; (H) Immunohistochemistry analysis of 8-OHdG intensity arbitrary in the peri-infarction cortex(number of animals: n=5/group). Scale bar, 50μm; (I) Quantitative analysis of protein levels of 8-OHdG by immunohistochemistry assay; (J–L) The mRNA levels of Caspase-3, Bax, and Bcl-2 were detected by qRT-PCR. Data were expressed as mean ± SD (number of animals: n =5/group); NS, not significant for p > 0.05, * p < 0.05, ** p < 0.01, and *** p < 0.001 (one-way analysis of variance with Tukey’s post hoc tests).