Research Paper Volume 16, Issue 3 pp 2828—2847

Figure 4. STAT3 overexpression promotes oxidative stress in OGD-treated neurons. The neurons were transfected with vector, STAT3(ORF), or co-transfected with vector and mimics NC, or co-transfected with vector and miR-124 mimics, or co-transfected with STAT3(ORF) and mimics NC, or co-transfected with STAT3(ORF) and miR-124 mimics. (A) The STAT3 mRNA was detected by qRT-PCR; (B) The protein levels of STAT3 were detected by Western blot; (C) Quantitative analysis of the protein levels of STAT3; (D) Intracellular ROS was measured using DCFH-DA with a fluorescence microscope; Representative photomicrograph of mito-tracker red; Representative photomicrographs of fluorescence shift from red to green of JC-1 staining. Scale bar, 50μm; (E) Quantitative analysis green fluorescence of ROS; (F) The SOD activity was determined by the commercial kits; (G) The content of MDA was determined by the commercial kits; (H) The content of 8-OHdG was determined by the commercial kits; (I) Quantitative analysis of mito-tracker red; (J, K) Quantitative analysis of fluorescence shift from red to green of JC-1 staining; Data were expressed as mean ± SD (derived from three independent experiments for each sample). NS, not significant for p > 0.05, * p < 0.05, ** p < 0.01, and *** p < 0.001 (one-way analysis of variance with Tukey’s post hoc tests).