Research Paper Volume 16, Issue 3 pp 2789—2811

Figure 4. Inhibiting PERK may suppress autophagy induced by MPPα-PDT, and enhance the anti-tumor ability of MPPα-PDT in HOS cells. HOS cells in MPPα-PDT+GSK2656157 group were pretreated with 5 mM GSK2656157 for 1 h before MPPα-PDT treatment. HOS cells in MPPα-PDT + Bafilomycin A1 group were pretreated with 100 nM bafilomycin A1 for 2 h before MPPα-PDT treatment. (A, B) Immunofluorescence analysis of p-PERK levels (magnification: ×400). (C–G) After indicated treatments, cells were harvested and PERK, p-PERK, ATF4, LC3-II/LC3-I, p62, cleaved caspase-3, and cleaved PARP levels determined by western blot. (H, I) Apoptotic rate was examined by flow cytometry. Data are shown as mean ± SD of 3 independent experiments. ^*P < 0.05.