Research Paper Volume 16, Issue 3 pp 2715—2735
Integrated single-cell and bulk characterization of branched chain amino acid metabolism-related key gene BCAT1 and association with prognosis and immunogenicity of clear cell renal cell carcinoma
Figure 3. Establishment of BCAA metabolism-related signature. (A) LASSO coefficient profiles of the expression of 9 prognostic BCAA metabolism-related genes. (B) Selection of the penalty parameter (λ) in the least absolute shrinkage and selector operation (LASSO) model via 10-fold cross-validation. (C) Heatmap showing the model gene expression and clinicopathological variables in the BCAA metabolism-related signature (BMS); (D) Risk curve of the risk score rank, scatter plot for the survival status distribution; (E) The KM survival curve for the survival probability of patients in different groups; (F) Receiver operating characteristic (ROC) curve in predicting 1-,2-, and 3-year overall survival (OS) based on BMS. (G, H) Univariate and multivariate cox regression analyses of clinicopathological variables for predicting the survival in ccRCC patients. (I) The KM survival curve of BCAA metabolism-related signature in the E-MTAB-1980 cohort. (J) ROC curve in predicting 1-,2-, and 3-year OS based on BMS in the E-MTAB-1980 cohort. (K) The KM survival curve of BCAA metabolism-related signature in the GSE22541 cohort. (L) ROC curve in predicting 1-,2-, and 3-year OS based on BMS in the GSE22541 cohort.