Research Paper Volume 16, Issue 3 pp 2320—2339

Figure 9. Inhibition of ALG3 decreased breast cell growth. (A) CCLE database demonstrated ALG3 expression levels in breast cancer cell lines. (B) The expression of ALG3 in breast cancer tissues on the level of protein. (C) RT-PCR and Western blot assays were used to examine si-RNA efficiency in breast cancer cells (left panel: RT-PCR; right panel: Western blot). (D) MTT assay was used to examine ALG3 down-regulation effect on breast cancer cells. (E) ALG3 down-regulation inhibited breast cancer cell colony formation ability. (F) Suppression of ALG3 contributed to the arrest of the cell cycle in the G1 and G2 phase. (G) Western blot assay was used to examine the expression levels of cyclinD1 and CDK4.