Research Paper Volume 16, Issue 2 pp 1968—1979

Figure 5. miR-6884-5p targeted S100A16 and regulated the expression of S100A16 in A549 cells. (A) The predicted binding sites of hsa-miR-6884-5p with wild-type 3′-UTR region of S100A16 mRNA are shown. A mutated 3′-UTR of S100A16 is also shown. (B) A549 cells were co-transfected with luciferase reporters containing Wt and/or mutant S100A16 3′-UTR with miR-6884-5p mimics and negative control. After 48 h of incubation, relative luciferase activities were measured. n = 3. Data were shown as means ± SD. **p < 0.01, Two-way ANOVA followed Turkey’s multiple comparisons test. C, A549 cells were transfected with miR-6884-5p mimics or negative control for 48 h. qRT-PCR was used to determine the expressions of miR-6884-5p (C) and the mRNA expressions of S100A16 (D). Western blot was used to determine the protein expressions of S100A16 (E). GAPDH was used as the loading control and the expressions were normalized to control (F). n = 3. Data were shown as Mean ± SD. **p < 0.01, ***p < 0.001 from Welch’s ANOVA test followed Dunnett’s T3 multiple comparisons test.