Research Paper Volume 16, Issue 2 pp 1938—1951

Figure 3. MiR-9-5p is also involved in proliferation and apoptosis of PCa cells. (A) The expression level of miR-9-5p was higher in PCa than in normal tissues in TCGA-PRAD. (B) The expression levels of miR-9-5p in four PCa cell lines (LNCaP, C4-2, PC3 and DU145) and normal cell line (RWPE-1). (C) The expression levels of miR-9-5p in 20 PCa tissues and paired normal tissues. (D) Western blot analysis revealed that the levels of cell apoptosis-related proteins (Bcl-2, cleaved-caspase3) and cell cycle-associated protein (Cyclin D1) were significantly altered after miR-9-5p regulated. (E) Immunofluorescence images of Ki-67 expression in LNCaP and PC3 cells with miR-9-5p downregulation or upregulation. Scale bar, 20μm. (F) Colony forming assay performed in LNCaP and PC3 cells to evaluate cell proliferation ability. (G, H) Cell viability of LNCaP and PC3 cells after knocking down or overexpressing lncMEG3 was determined using CCK8 assays. EdU assays also implicated the same results. (I, J) Cell cycle distribution and apoptosis were analysed by flow cytometry in LNCaP and PC3 cells, * P < 0.05.