Research Paper Volume 16, Issue 2 pp 1938—1951

Figure 1. LncMEG3 was downregulated and indicated a poor clinical prognosis in PCa. (A) Differential genes between carcinoma and paracancerous tissues in patients with PCa (n=5). (B) The expression level of lncMEG3 was lower in PCa than in normal tissues in TCGA-PRAD and GTEx database. (C) LncMEG3 show a significant differences in cancerous and paracancerous tissues (GSE88808). (D) The expression level of lncMEG3 exhibit a declining tendency with the progression of PCa (GSE6919). (E) The expression levels of lncMEG3 in 20 PCa tissues and paired normal tissues. (F) The expression levels of lncMEG3 in four PCa cell lines (LNCaP, C4-2, PC3 and DU145) and normal cell line (RWPE-1). (G) Localisation of lncMEG3 was assessed by PCR in PC3 cell line. U6 and GAPDH were used as positive controls for nuclear RNA and cytoplasmic RNA, respectively. (H, I) The distribution of lncMEG3 was also analysed by FISH in PC3 cells (Scale bar, 20μm) and PCa tissues (Scale bar, 50μm). 18S showed in the cytoplasm. (J) The patients with lower level of lncMEG3 were more likely to have biochemical recurrence according to the dates from GSE70770, * P < 0.05.