Research Paper Volume 16, Issue 2 pp 1925—1937

Figure 4. MDM2 regulates the ubiquitination of ACSL4 protein. (A) The half-life of ACSL4 protein in CHX chase assay was found to be increased by si-MDM2, as shown by WB analysis. (B) Overexpression of MDM2 resulted in an increase in polyubiquitinated ACSL4 in HT-22 cells, as observed through IP-WB analysis. (C) Knockdown of MDM2 led to a decrease in polyubiquitinated ACSL4 in HT-22 cells, as demonstrated by IP-WB analysis. Data are presented as mean ± SD. ^*P < 0.05 vs. NC or si-NC group.