Research Paper Volume 16, Issue 2 pp 1897—1910
Knockdown of RNA-binding protein IMP3 suppresses oral squamous cell carcinoma proliferation by destabilizing E2F5 transcript
Figure 5. Effects of E2F5 inhibition on OSCC cell growth. The expression of E2F5 was analyzed with qRT-PCR (A) and Western blot (B) after transfection with siE2F5 and its control. (C) Cell viability of SCC-25 and SCC-4 cells transfected with siE2F5 was determined by the CCK8 assay (n=5). (D) Effects of suppression of E2F5 on the cell cycle progression of OSCC cells measured by flow cytometric analysis. (E) Expression analysis for cell cycles regulators Cyclin D1 and CDK4 was performed by Western blot. Relative expression levels were calculated using the image J software (n = 3). P ≤ 0.05 was considered to be statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001.