Research Paper Volume 16, Issue 2 pp 1897—1910
Knockdown of RNA-binding protein IMP3 suppresses oral squamous cell carcinoma proliferation by destabilizing E2F5 transcript
Figure 2. Knockdown of IMP3 inhibits OSCC proliferation. The expression of IMP3 in SCC-25 and SCC-4 cells transfected with IMP3 siRNA was determined by qRT-PCR (A) and Western blot (B). (C) Cell growth in OSCC cells transfected with siIMP3 as measured by CCK8 assay(n=5). (D) Effects of suppression of IMP3 on the cell cycle progression of SCC-25 and SCC-4 cells measured by flow cytometric analysis. (E) Expression analysis for cell cycles regulators Cyclin D1 and CDK4 was performed by Western blot. Relative expression levels were calculated using the image J software (n = 3). P ≤ 0.05 was considered to be statistically significant, **P < 0.01, ***P < 0.001, ****P < 0.0001.