Research Paper Volume 16, Issue 2 pp 1808—1828
Identification of BANF1 as a novel prognostic biomarker in gastric cancer and validation via in-vitro and in-vivo experiments
Figure 8. Knockdown of BANF1 inhibits the proliferation, migration and invasion ability of GC cells in vitro. (A) Knockdown efficiency of MKN-45 cell line was detected using RT-qPCR and WB. (B) CCK8 assay was used to detect the effect of BANF1 knockdown on the viability of MKN-45 cell line. (C) Knockdown efficiency of BGC-823 cell line was detected using RT-qPCR and WB. (D) CCK8 assay was used to detect the effect of BANF1 knockdown on the viability of BGC-823 cell line. (E, F) The effect of BANF1 knockdown on the proliferative capacity of MKN-45 and BGC-823 cells was detected by plate clone formation assay. (G, H) Effects of BANF1 knockdown on the migration ability of MKN-45 and BGC-823 cells were detected by wound healing assay. (I, J) Effect of BANF1 knockdown on the migration and invasion of MKN-45 and BGC-823 cells by Transwell assay. All experiments were repeated at least three times. ***p < 0.001.