Research Paper Volume 16, Issue 2 pp 1808—1828

Figure 7. Expression and localization of BANF1. (A) BANF1 expression among pan-cancer unpaired samples in the TCGA database. (B) BANF1 expression among paired samples of pan-cancer in the TCGA database. (C) RT-qPCR detection of BANF1 mRNA expression in 23 pairs of GC tissues and paired paracancerous tissues. (D) WB detection of BANF1 protein expression in 10 pairs of GC tissues, and the difference of gray values between gastric cancer tissues (n = 10) and adjacent tissues (n = 10) were compared. (E) RT-qPCR was used to detect the expression of BANF1 mRNA in GC cell lines (AGS, HGC-27, MKN-45, MGC-803, BGC-823) and gastric mucosal epithelial cell line (GES-1), respectively. (F) The expression of BANF1 protein in 6 cell lines was detected by WB, and the gray values of gastric cancer cell lines (AGS, HGC-27, MKN-45, MGC-803, and BGC-823) and gastric epithelial cells (GES-1) were compared. (G) Immunofluorescence staining showed the expression and localization of BANF1 protein in MKN-45 and BGC-823 cells. All experiments were repeated at least three times. ns means no statistical difference, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.