Research Paper Volume 16, Issue 2 pp 1781—1795
Identification of key ferroptosis genes and mechanisms associated with breast cancer using bioinformatics, machine learning, and experimental validation
Figure 5. Transfection efficiency detection. (A) q-PCR analysis of the transfection efficiency of siRNA-SLC2A1, TXNIP overexpression plasmids, and ATF3 overexpression plasmids. (B–E) Western blot analysis of the transfection efficiency of siRNA-SLC2A1, TXNIP overexpression plasmids, and ATF3 overexpression plasmids. The bar graphs show the mean ± SD of at least three independent experiments (n = 3). **P < 0.01, compared to the Control group, the difference is extremely significant.