Research Paper Volume 16, Issue 2 pp 1767—1780
Tremella fuciformis polysaccharides induce ferroptosis in Epstein–Barr virus-associated gastric cancer by inactivating NRF2/HO-1 signaling
Figure 5. TFP suppressed NRF2/HO-1 activation in both EBV-infected AGS and MKN45 cells. EBV-infected AGS and MKN45 cells were preincubated with 40 μg/mL or 20 μg/mL TFP or 10 μM erastin for 24 h. (A) DCFH-DA staining showed that TFP elevated ROS production in EBV-infected AGS and MKN45 cells (bar=30 μm). Western blot assays showed that TFP significantly suppressed the expression of NRF2, HO-1, GPX4 and xCT in both AGS (B) and MKN45 (C) cells. *p<0.05, **p<0.01, ***p<0.001 vs. DMSO; #p<0.05, ##p<0.01 vs. erastin.