Research Paper Volume 16, Issue 2 pp 1767—1780

Figure 4. TFP induced ferroptosis in EBV-infected AGS and MKN45 cells. EBV-infected AGS and MKN45 cells were preincubated with 1 μM Fer-1, 20 μM Z-VAD-FMK, 20 μM Nec-1 and 20 μM 3-MA for 1 h and then treated with TFP for 24 h. CCK-8 assays showed that TFP elevated EBV-infected AGS (A) and MKN45 (B) cell death, but preincubation with Fer-1 reduced these effects. RT–PCR analysis indicated that the mRNA levels of Ptgs2 and Chac1 were increased after TFP treatment, but Fer-1 preincubation abolished these effects in AGS (C) and MNK45 (D) cells. TFP enhanced the levels of Fe²⁺ (E) and MDA (F) in EBV-infected AGS and MKN45 cells, but Fer-1 preincubation abolished these effects. (G) TFP-induced reduction of GSH could also be abolished by Fer-1 in both EBV-infected AGS and MNK45 cells. **p<0.01, ***p<0.001 vs. con; ##p<0.01, ###p<0.001 vs. TFP.