Research Paper Volume 16, Issue 2 pp 1767—1780

Figure 1. EBV-infected AGS and MKN45 cells are sensitive to erastin-induced ferroptosis. EBV-infected AGS and MKN45 cells were preincubated with 1 μM Fer-1 for 1 h and then treated with or without 10 μM erastin for 24 h. (A) CCK-8 assay demonstrated that erastin reduced EBV-infected AGS and MKN45 cell viability, but Fer-1 partially abolished these effects. The erastin-induced upregulation of MDA (B) and Fe²⁺ (C) levels was reversed by Fer-1 pretreatment in both EBV-infected AGS and MKN45 cells. (D) Erastin decreased intracellular GSH levels, but Fer-1 elevated GSH levels in EBV-infected AGS and MKN45 cells. ^*p<0.05, ^**p<0.01, ^***p<0.001 vs. DMSO; ^#p<0.05, ^##p<0.01, ^###p<0.001 vs. erastin.