Research Paper Volume 16, Issue 2 pp 1463—1483
Identification and validation of anoikis-related lncRNAs for prognostic significance and immune microenvironment characterization in ovarian cancer
Figure 8. Validation of the expression and function of the five anoikis-related lncRNAs in ovarian cancer cell lines. (A) RT-PCR detection of the expression levels of PRR34_AS1, LINC01094, SPAG5_AS1, AC106801.1, and CACNA1G_AS1 lncRNAs in various ovarian cancer cell lines (Caov3, HO-8910PM, OVCAR3, SKOV3) and normal ovarian cells (HOSEpiC). (B) Validation of the relative RNA expression in SKOV3 and OVCAR3 cells after knockdown of PRR34_AS1 or SPAG5_AS1. (C) Flow cytometry analysis of the proportion of apoptotic cells in SKOV3 and OVCAR3 cells after knockdown of PRR34_AS1 and SPAG5_AS1, respectively. (D) Fluorescent image showing live/dead SKOV3 and OVCAR3 cells after knockdown of PRR34_AS1 or SPAG5_AS1 using calcein-M and PI staining (living cells in green fluorescence, dead cells in red fluorescence).