Figure 3. Effects of GAN on tumor-bearing mice. (A–C) M1 markers (n = 8). GAN produced unobvious effects on M1 markers, without showing inter-group differences. (D–F) M2 markers (n = 8). GAN could inhibit the expressions of M2 markers, showing significant differences from the Control. (G) IHC (n = 5). Expressions of CD206 were strongly positive in Control. (H, I) Relative protein expressions (n = 5). GAN could inhibit the phosphorylated expression of STAT6, leading to significantly lower p-STAT6 level than that in Control. *P < 0.05 vs. control.