Research Paper Volume 16, Issue 2 pp 1352—1373

Figure 6. H/R rat model construction and in vivo validation of miR-141-3p/SIRT1/Beclin-1 axis regulatory function. (A) Dry/wet weight ratio of lung tissue of sham-operated rats and H/R rats. (B) HE staining for detecting the lung tissue injury of sham-operated and H/R rats. (C) TUNEL staining for detecting the apoptosis of lung tissue in sham-operated and H/R rats. (D) The level of miR-141-3p in lung tissue of sham-operated rats and H/R rats detected by RT-qPCR. (E) Western blot to detect SIRT1, Beclin-1, LC3-II/LC3-I levels in lung tissue of sham-operated and H/R rats. (F) Dry/wet weight ratio of lung tissue of H/R rats in response to miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. (G) HE staining to detect the lung tissue injury of H/R rats in response to miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. (H) TUNEL staining to detect the apoptosis of lung tissue in H/R rats in response to miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. (I) The level of miR-141-3p in lung tissue of H/R rats in response to miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1 detected by RT-qPCR. (J) Western blot to detect the levels of SIRT1, Beclin-1 and LC3-II/LC3-I in lung tissue of H/R rats in response to miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. n = 5. Measurement data are expressed as mean ± standard deviation. Changes between multiple groups were compared using one-way ANOVA and Tukey’s multiple comparison test. Two-way ANOVA was used for data comparison at different time points. * p < 0.05.