Research Paper Volume 16, Issue 2 pp 1352—1373

Figure 5. miR-141-3p mimic suppressed PMVEC autophagy and injury via the SIRT1/Beclin-1 axis. (A) The expression of miR-141-3p detected by RT-qPCR in H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. (B) The expression of SIRT1, Beclin-1, LC3-I/LC3-II detected by Western blot in H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. (C) mRFP-GFP-LC3 dual fluorescence system was used to trace autophagy formation in H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1. (D) The proliferation of H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1 detected by CCK-8 assay. (E) The migration of H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1 detected by Transwell assay. (F) The apoptosis of H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1 detected by flow cytometry. (G) The expression of Bax, Bcl-2, cleaved caspase-3 and caspase-3 in H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with oe-SIRT1 or oe-Beclin-1 detected by Western blot. Measurement data are expressed as mean ± standard deviation. Changes between multiple groups were compared using one-way ANOVA and Tukey’s multiple comparison test. Two-way ANOVA was used for data comparison at different time points. * p < 0.05. The cell experiments were repeated three times.