Research Paper Volume 16, Issue 2 pp 1336—1351
Cisplatin and doxorubicin chemotherapy alters gut microbiota in a murine osteosarcoma model
Figure 1. CDDP and DOX inhibit the osteosarcoma tumor growth in mice. (A) Outline of the experimental procedure. (B) Photographs of osteosarcoma tumor xenografts. Saos-2 cells were injected into nude mice, which were then treated with normal saline, CDDP, or DOX. After 20 days, osteosarcoma xenograft tumors were completely dissected and photographed. (C) Weight of the tumors. (****P < 0.0001; ns P > 0.05; n = 6). (D) Immunohistochemistry: Immunohistochemistry analyses were performed on mouse osteosarcoma xenograft tumor tissues. (E) The percentage of Ki67+ cells in the tumors. (***P < 0.001; ns P > 0.05; n = 6) Scale bar, 100 μm. Abbreviations: CDDP: cisplatin; DOX: doxorubicin.