Research Paper Volume 16, Issue 1 pp 844—856
Melatonin regulates autophagy in granulosa cells from patients with premature ovarian insufficiency via activating Foxo3a
Figure 2. Attenuated autophagy in GCs from POI patients. (A) mRNA levels of autophagy-related genes in GCs (n = 4-6). (B) Representative Western blots (WB) and analysis of autophagy-related proteins in GCs (n = 3). (C) Representative IF staining and analysis of autophagy flux in GCs (n = 5). Scale bar, 50 μm. ns, no significant, *p < 0.05, **p < 0.01, ***p < 0.001.