Research Paper Volume 16, Issue 1 pp 844—856
Melatonin regulates autophagy in granulosa cells from patients with premature ovarian insufficiency via activating Foxo3a
Figure 1. Morphological and functional characteristics of granulosa cells (GCs) in premature ovarian insufficiency (POI) patients and normal controls. (A) Microscopic images depicting the morphology of GCs. Scale bar, 50 μm. (B) Representative immunofluorescence (IF) staining and analysis of cell proliferation in GCs (n = 5). Scale bar, 100 μm. (C) Flow cytometry analysis of apoptotic rates in GCs (n = 3). (D) Analysis of reactive oxygen species (ROS) production in GCs. **p < 0.01, ***p < 0.001.