Research Paper Volume 16, Issue 1 pp 779—798
SPC25 as a novel therapeutic and prognostic biomarker and its association with glycolysis, ferroptosis and ceRNA in lung adenocarcinoma
Figure 3. The impact of knocking down SPC25 on LUAD. (A, B) An analysis of Western blotting confirmed the success of SPC25-siRNAs transfection into H1975 cells and their ability to interfere with gene expression. (C, D) CCK-8 assay evaluated tumor cell proliferation activity. (E, F) EdU staining of H1975 cells and EdU-positive cell proportion. (G, H) The effect of SPC25 knockdown on apoptosis of the H1975 cells as detected by flow cytometry. (I–K) Representative images and quantitative analysis of wound healing measurements in LUAD cells. (***p < 0.001; **p < 0.01; *p < 0.05; Abbreviation: ns: not significant).