Figure 9. Effects of PTGS2 knockdown on oxidative stress and ferroptosis of IS in vitro. (A) ROS level of primary neuronal cells. (B) MDA level of primary neuronal cells. (C) GSH level of primary neuronal cells. (D) Oxidative stress index (OSI) level of primary neuronal cells. (E) Iron level of primary neuronal cells. (F, G) RT-qPCR analysis of SLC7A11 and GPX4 mRNA level. (H–J) Western blot analysis of SLC7A11 and GPX4 protein level. *p < 0.05; **p < 0.01; ***p < 0.001.