Figure 7. OGT knockdown inhibits the migration and invasion capability of osteosarcoma cells. (A) OGT expression in normal bone tissues and osteosarcoma tissues (GSE16088 and GSE28425). (B) OGT mRNA expression level in osteosarcoma cells. (C) OGT knockdown in MG63 and U2OS cells was confirmed using RT-PCR and WB. (D, E) Migration and invasion capability of MG63 and U2OS cells were significantly weakened by the downregulation of OGT expression (**p <0.01, ***p <0.001). (F) KEGG pathway analysis of OGT in osteosarcoma.