Research Paper Volume 16, Issue 1 pp 648—664
Danggui-Shaoyao-San (DSS) ameliorates the progression of osteoarthritis via suppressing the NF-κB signaling pathway: an in vitro and in vivo study combined with bioinformatics analysis
Figure 6. DSS suppressed ECM degradation in rat chondrocytes. The chondrocytes were pretreated with DSS for 24 h and stimulated with or without LPS for 24 h. (A, B) The mRNA expression levels of MMP3 and MMP13 were detected by qRT-PCR. (C) The protein levels of MMP3 and MMP13 in chondrocytes were measured by western blot. (D) Representative immunofluorescence image of collagen II, and the fluorescence intensities were quantified by Image J. ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. control group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. LPS alone treatment group, n = 3.