Research Paper Volume 16, Issue 2 pp 1237—1248
Silencing of METTL3 prevents the proliferation, migration, epithelial-mesenchymal transition, and renal fibrosis of high glucose-induced HK2 cells by mediating WISP1 in m6A-dependent manner
Figure 4. METTL3 silencing prevented EMT progression and fibrosis of HG-induced HK2 cells by decreasing WISP1 with m6A modification. (A) MeRIP-qPCR assay exhibited the change in WISP1 enrichment in HG-treated HK2 cells after METTL3 silencing. (B) WISP1 expression was examined via qRT-PCR in HG-treated HK2 cells which were transfected with METTL3 sh-RNAs. (C) HG-treated HK2 cells were transfected with sh-METTL3 or/and OE-WISP1, and the cell proliferation was tested by CCK-8 assay. (D) Wound healing assay showed the change of cell migration in the processed HK2 cells after HG treatment. (E) E-cadherin, Fibronectin and a-SMA expression levels were assessed by applying western blot. (F) Western blot was adopted to monitor the changes of c-MYC, Wnt1 and β-catenin expressions. **P < 0.01, ***P < 0.001.