Research Paper Volume 16, Issue 2 pp 1237—1248
Silencing of METTL3 prevents the proliferation, migration, epithelial-mesenchymal transition, and renal fibrosis of high glucose-induced HK2 cells by mediating WISP1 in m6A-dependent manner
Figure 2. The impacts of WISP1 on the proliferation, migration, EMT, and renal fibrosis of HG-treated HK2 cells. (A) After HG treatment, the expression of WISP1 was confirmed using qRT-PCR and western blot in HK2 cells transfected with NC or sh-WISP1. (B) QRT-PCR and western blot analysis of WISP1 in HK2 cells transfected with OE-WISP1 or Vector. (C) CCK-8 assay for the assessment of cell proliferation in HG-induced HK2 cells with WISP1 silencing and HK2 cells with WISP1 overexpression. (D) Wound healing assay was conducted to identify the migration ability of the WISP1-silenced HG-induced HK2 cells and WISP1-overexpressed HK2 cells. (E) Western blot indicated the expression changes of E-cadherin, Fibronectin and a-SMA in WISP1-silenced HG-induced HK2 cells and WISP1-overexpressed HK2 cells. (F) The expressions of c-MYC, Wnt1 and β-catenin were monitored by applying western blot in each group. *P < 0.05, **P < 0.01.