Research Paper Volume 16, Issue 2 pp 1237—1248
Silencing of METTL3 prevents the proliferation, migration, epithelial-mesenchymal transition, and renal fibrosis of high glucose-induced HK2 cells by mediating WISP1 in m6A-dependent manner
Figure 1. WISP1 expression was highly expressed in DN model mice and HG-treated HK2 cells. DN model mice were first established using streptozotocin. (A) 24-h urinary protein. (B) qRT-PCR analysis of WISP1 expression in kidney tissues which were removed from normal and DN mice (n=5). (C) QRT-PCR analysis of WISP1 expression in HG-treated HK2 cells. (D) The protein level expression of WISP1 in renal tissues and HK2 cells. (E) The total m6A RNA methylation context of total RNA between control and DN group. (F) The total m6A RNA methylation context of total RNA between control and HG group. *P < 0.05, **P < 0.01.