Research Paper Volume 15, Issue 24 pp 15504—15524

Figure 2. Basic information of VRK1 expression. (A) The different VRK1 expressions of the 31 types of cancers. (B) The different VRK1 expression in the cancer cell lines. (C) Comparison of VRK1 mRNA levels between cancerous and adjacent normal tissue from TCGA and GTEx datasets. (D) The mRNA level of VRK1 between LIHC and normal tissues. (E) The protein expression level of VRK1 between LIHC and normal tissues. (F, G) Western blotting and qRT-PCR assays explored the protein and mRNA expression level of VRK1 in the LIHC tissues and paired normal tissues. GAPDH was used as a loading control. (H, I) The expression level of VRK1 was detected using public databases and collected liver cancer tissue specimens by using IHC assay. (J) The immunofluorescence images of VRK1 protein, nucleus, endoplasmic reticulum (ER), microtubules, and the incorporative images in A-431, U-251MG, and U2-OS cell lines. (K) Protein–protein interactions of diagram of VRK1 protein (^*P < 0.05, ^**P < 0.01, and ^***P < 0.001).