Research Paper Volume 15, Issue 24 pp 15489—15503

Figure 4. Verification of the the eccDNA ‘junctional and motif analysis of all eccDNA’ junctional sites. (A) Agarose gel electrophoresis of PCR products from eccDNA. (B) Base sequence of the junctional sites we obtained from eccDNA sequencing and Sanger sequencing. The arrow indicates the junction. (C) Motif analysis of the junctional sites obtained from the high-throughput sequencing. Consecutive represents ≥three consecutive identical bases at the start and end positions of eccDNA molecules with 4 bp spacers in between.