Figure 4. The neuronal fiber density in different regions of the brain was evaluated with the immunofluorescent MAP2-labeled staining. (A) CA1, (B) CA3, (C) DG, (D) Cortex, (E) Striatum regions were stained with MAP2 antibody and DAPI. The green and blue staining indicated the MAP2-labeled neuronal fibers and the nucleus, respectively. (F) The density of MAP2-labeled neuronal fibers in different regions. Scale bar = 25μm. The scale bar in the magnified image = 5μm. [(Con group, n=3), (2VO group, n=3)].