Research Paper Volume 16, Issue 1 pp 493—517

Figure 10. Verification of in vitro experiment about the function of the key gene SNHG6 in osteosarcoma cells. (A, B) The levels of SNHG6 mRNA in 143B and MG63 cells that were transfected with si-SNHG6 were measured using qRT-PCR (*** P ≤ 0.001). (C, D) The viability of 143B and MG63 cells with SNHG6 knockdown was found to be reduced according to the CCK-8 assay (* P ≤ 0.05). (E, F) The ability of 143B and MG63 cells to form colonies was significantly reduced after SNHG6 knockdown (*** P ≤ 0.001). (G, H) The migration and invasion of 143B and MG63 cells were significantly inhibited after SNHG6 knockdown (*** P ≤ 0.001). (I) The impact of SNHG6 knockdown on the AXL mRNA level in 143B cells was assessed using qRT-PCR (*** P ≤ 0.001). (J) WB analyzed the molecular weight of AXL and p-AXL in 143B cell with SNHG6 knockdown. (K) WB analyzed the molecular weight of AKT and p-AKT in 143B cell with SNHG6 knockdown. (L) The impact of SNHG6 knockdown on the AXL mRNA level in MG63 cells was assessed using qRT-PCR (*** P ≤ 0.001). (M) WB analyzed the molecular weight of AXL and p-AXL in MG63 cell with SNHG6 knockdown. (N) WB analyzed the molecular weight of AKT and p-AKT in MG63 cell with SNHG6 knockdown.