Research Paper Volume 16, Issue 1 pp 466—492
G6PD is a prognostic biomarker correlated with immune infiltrates in lung adenocarcinoma and pulmonary arterial hypertension
Figure 10. Identification of G6PD mRNA expression in LUAD and PAH. (A) The expression of G6PD in the tumors and lung organs through immunofluorescence imaging (n = 3). (B) The expression of G6PD in hypoxia-induced PAH tissues and normal lung tissue through immunofluorescence imaging (n = 3). (C, D) G6PD expression levels in normal cells and hypoxia cells (C), and LUAD four cell lines (D). (E) qRT-PCR analysis of G6PD expression in HPASMC transduced with siRNA. (F) EDU assay in transduced hypoxia cells. (G) CCK8 assay in transduced hypoxia cells. (H) qRT-PCR analysis of G6PD expression in A549 cell transduced with siRNA. (I) Colony formation assay and transwell assay of migration/invasion ability in transduced A549 cell.