Research Paper Volume 15, Issue 24 pp 15451—15472
Identification and validation of key biomarkers associated with macrophages in nonalcoholic fatty liver disease based on hdWGCNA and machine learning
Figure 10. Validation of optimal feature genes in mouse module. (A) Fresh Livers and liver-to-body weight ratio in control and MCD mice. (B) The serum ALT and AST levels on control and MCD mice. (C) HE staining and Oil Red O staining of liver sections from mice fed on control or MCD-diet. (D) The relative expressions of MAFB and CX3CR1 were validated by RT-qPCR. (E) MAFB and CX3CR1 expression in liver tissues of control and MCD mice was detected by IHC. (F) MAFB and CX3CR1 expression in liver tissues of control and MCD mice was detected by WB. ***P < 0.001, ****P < 0.0001.