Research Paper Volume 15, Issue 23 pp 14457—14472
MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression
Figure 7. miR-203a-3p alleviated the destruction of articular cartilage in ACLT rats. (A) ACLT rat model and miR-203a-3p agomir treatment protocol. (B) Safranin O-Fast Green, HE, and Toluidine blue staining of rat knee joints were performed to assess the histological morphology of the different groups at 8 weeks after miR-203a-3p agomir treatment. (C) The OARSI scores of the different groups were calculated respectively at 8 following after miR-203a-3p agomir treatment. (D, E) Immunofluorescence staining of Collagen II (green) and MMP13 (red) were performed in rat articular cartilage at 8 weeks following miR-203a-3p agomir treatment. All experiments were performed in triplicated and data were presented as the mean±SD, n=3 per group. *P<0.05, **P<0.01.