Research Paper Volume 16, Issue 1 pp 299—321
METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes
Figure 8. Berberine regulates METTL3-mediated m6A modification of NEAT1 to alleviate ischemic stroke in adult mouse astrocytes. (A) Western blot to verify METTL3 expression in adult mouse astrocytes after berberine administration following MCAO/R. (B) A bar presenting the quantification of METTL3. (C) RT-qPCR for NEAT1 expression in adult mouse astrocytes after berberine administration following MCAO/R. (D) RT-qPCR to verify the expression of miR-377-3p in adult mouse astrocytes after berberine administration following MCAO/R. The relative expression levels were quantified by normalizing to β-actin. Data are represented as mean ± SD, (n = 6; **P < 0.01; ***P < 0.001).