Research Paper Volume 16, Issue 1 pp 299—321
METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes
Figure 4. NEAT1 binds to miR-377-3p in OGD/R astrocytes and plays a neuronal protective role as a ceRNA. (A) Prediction of NEAT1 binding sites on miR-377-3p using bioinformatics software (RNAhybrid). (Schematic diagram of the sequence complementation relationship of miR-377-3p in NEAT1.) (B) Dual luciferase reporter gene analysis to confirm the binding relationship between NEAT1 and miR-377-3p. (C) RT-qPCR to analyze the NEAT1 level in primary astrocytes transfected with sh-NEAT1 or Lenti- NEAT1. (D) RT-qPCR to analyze the miR-377-3p level in primary astrocytes transfected with sh-NEAT1 or Lenti- NEAT1. (E) CCK-8 assay to assess the co-cultured neuron viability after NEAT1 knockdown or overexpression in primary astrocytes and OGD/R treatment. (F) LDH assay to study the effect of the co-cultured neuron viability after NEAT1 knockdown or overexpression in primary astrocytes and OGD/R treatment. Data are represented as mean ± SD, (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001).