Research Paper Volume 16, Issue 1 pp 299—321
METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes
Figure 3. Berberine regulates Nampt expression through downregulation of miR-377-3p in OGD/R-treated primary astrocytes, thereby affecting neuronal activity. (A) Using RT-qPCR to analysis the miR-377-3p level in cells transfected with miR-377-3p mimic after berberine treatment. (B) Using Western blot to analyze Nampt expression in astrocytes after berberine treatment and transfection with miR-377-3p mimic. (C) A bar presenting the quantification of Nampt in primary astrocytes. (D) Using the CCK-8 assay to evaluate the effect of co-culture with berberine and primary astrocyte transfected with miR-377-3p mimic on neurons after OGD/R. (E) Using LDH assay to study the effect of co-culture with berberine and primary astrocyte transfected with miR-377-3p mimic on neurons after OGD/R. The relative expression levels were quantified by normalizing to β-actin. Data are represented as mean ± SD, (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001).