Research Paper Volume 16, Issue 1 pp 191—206

Figure 6. Mn²⁺ and IL-12 combination treatment promoted the conversion of immune-exclusion tumors into immune-inflamed tumors and enhanced immune cell infiltration in vivo. (A, B) Representative images of costimulatory CD80 and CD86 staining in the tumor center. (C) Representative images of M1-type macrophages co-localized by F4/80 (red) and iNOS (green) in the tumor center. (D) Cytokine levels in the circulation of tumor-bearing mice. (E, F) Representative images of CD4 and CD8 staining in the tumor center (scale bar, 50 μm). Abbreviation: NS: not significant. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.