Research Paper Volume 15, Issue 24 pp 15360—15381
Clinical and therapeutical significances of the cluster and signature based on oxidative stress for osteosarcoma
Figure 7. Importance of RHBDL2 in TARGET-OS cohort. (A) Kaplan-Meier survival curve showing survival probability of high- or low-expression RHBDL2; (B) The 1-year, 2-year, 3-year, 4-year, and 5-year survival ROC curves predicted by the expression of RHBDL2, with curves in different colors representing the AUC for different years; (C) The forest figure for Univariate and Multivariate Cox regression analyses of RHBDL2 and clinicopathologic features (Age, Sex); (D) The relationship of RHBDL2 with immune checkpoints in TARGET cohort; (E) Quantitative Real-time PCR. (A–C) Quantitative Real-time PCR assays using cell lines for RHBDL2; (F) The protein expression levels of ENO1 and CD44 in HK-2 cell control group and hyperglycemic group by Western blot; (G) The detection of RHBDL2 in tumor cells and normal cells by immunofluorescence.