Research Paper Volume 16, Issue 1 pp 106—128

Figure 4. The effects of JPYSF on fibrotic response in TGF-β1-induced HK-2 cells. (A) Representative Western blot images of α-SMA and vimentin expression in HK-2 cells stimulated with TGF-β1 at the concentrations of 0, 2, 5, and 10 ng/mL. (B, C) Quantitative analysis of α-SMA and vimentin expression in HK-2 cells stimulated with TGF-β1 at the concentrations of 0, 2, 5, and 10 ng/mL, normalized to α-Tubulin or β-actin content (n = 3). (D) Cell viability rate (%) (n = 3). (E) Representative Western blot images of α-SMA and vimentin expression in HK-2 cells stimulated with TGF-β1 or/and JPYSF at the concentrations of 0.5 and 1 mg/mL. (F, G) Quantitative analysis of α-SMA and vimentin expression in HK-2 cells stimulated with TGF-β1 or/and JPYSF at the concentrations of 0.5 and 1 mg/mL, normalized to GAPDH content (n = 3). (H) Representative immunofluorescence images of α-SMA and vimentin in HK-2 cells with TGF-β1 or/and 1 mg/mL JPYSF stimulation. Green corresponds to interest proteins, and blue corresponds to nuclear staining. All images are shown at identical magnification, ×400, scale bar = 50 μm. Data are expressed as mean ± SEM (^nsp > 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 between the indicated two groups).